cell culture mcf 7  (ATCC)

Journal: Molecular Biology Reports

Article Title: SUMOylation machinery protein, PIAS4 role in breast cancer cell proliferation and drug sensitivity

doi: 10.1007/s11033-025-11423-0

Figure Lengend Snippet: SUMOylation genes expression levels in human breast cells and naked mole rat intestinal tissues. Gene expression in MCF-10-A (control breast), MCF-7 cell lines, and NMR intestinal tissue. (a) SENP isoforms (b) SUMO1, SUMO2, and SUMO3 (c) SAE1, UBA2, and UBE21 (d) PIAS isoforms gene expression. qPCR was used to confirm SUMOylation gene expression levels. Mean and Error bars correspond to SD from three repeats of individual experiments ( n = 3). Statistical analysis was performed by ordinary two-way ANOVA and indicated a significant difference in where *indicates p < 0.05, **indicates p < 0.01, ***indicates p < 0.001, and ****indicates p < 0.0001

Article Snippet: Non-malignant breast epithelial cells (MCF-10 A) at passage 3 (P3) and human breast cancer cells (MCF-7) at passage 6 (P6) were provided by the Institute of Cancer Therapeutics (ICT) at the University of Bradford (UoB) originally sourced from ATCC.

Techniques: Expressing, Gene Expression, Control

Journal: Molecular Biology Reports

Article Title: SUMOylation machinery protein, PIAS4 role in breast cancer cell proliferation and drug sensitivity

doi: 10.1007/s11033-025-11423-0

Figure Lengend Snippet: DOX treatment and PIAS4 overexpression affects protein expression levels in MCF-7 cells. (a) PIAS4 protein expression in MCF-10-A cells and NMR intestinal tissue, (b) Histogram analysis of (a). (c) PIAS4 protein expression in MCF-10-A and MCF-7 cells. (d) Histogram analysis of ( c ). (e) PIAS4 and Bcl2 protein expression levels in MCF-7 cells stably expressing Exp.dTomato (control) or Exp.PIAS4, with or without DOX treatment. ( f ) Histogram of PIAS4 expression levels from ( e ). (g) Histogram of Bcl-2 protein levels from ( e ). All Western blots were derived from the same lysate, performed concurrently, and represent three independent experiments ( n = 3). Protein expression levels were normalised either α-Tubulin, GAPDH or β-actin. Data are presented as mean ± standard deviation (SD) from three independent experimental repeats. Statistical analysis was performed using one-way ANOVA. * p < 0.05, *** p < 0.001, **** p < 0.0001 indicate statistically significant differences

Article Snippet: Non-malignant breast epithelial cells (MCF-10 A) at passage 3 (P3) and human breast cancer cells (MCF-7) at passage 6 (P6) were provided by the Institute of Cancer Therapeutics (ICT) at the University of Bradford (UoB) originally sourced from ATCC.

Techniques: Over Expression, Expressing, Stable Transfection, Control, Western Blot, Derivative Assay, Standard Deviation

Journal: Molecular Biology Reports

Article Title: SUMOylation machinery protein, PIAS4 role in breast cancer cell proliferation and drug sensitivity

doi: 10.1007/s11033-025-11423-0

Figure Lengend Snippet: Functional effects of PIAS4 overexpression in MCF-7 cells with and without DOX treatment. (a) IC₅₀ of 48-hour DOX treatment in MCF-7 cells transfected with Exp.dTomato or Exp.PIAS4. MCF-7 cells stably expressing Exp.dTomato or Exp.PIAS4 plasmids following 48-hour DOX treatment (b) Cell invasion was assessed using fluorescence measurements and reported as relative fluorescence units (RFU) at 480/520 nm. (c) Colony formation images were analysed using ImageJ ® software for quantification. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical analysis was performed in GraphPad Prism using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Non-malignant breast epithelial cells (MCF-10 A) at passage 3 (P3) and human breast cancer cells (MCF-7) at passage 6 (P6) were provided by the Institute of Cancer Therapeutics (ICT) at the University of Bradford (UoB) originally sourced from ATCC.

Techniques: Functional Assay, Over Expression, Transfection, Stable Transfection, Expressing, Fluorescence, Software

Journal: Molecular Biology Reports

Article Title: SUMOylation machinery protein, PIAS4 role in breast cancer cell proliferation and drug sensitivity

doi: 10.1007/s11033-025-11423-0

Figure Lengend Snippet: Schematic diagram for the PIAS4-mediated mechanisms in MCF-7 breast cancer cells. The increased level of PIAS4 in the naked mole-rat intestine, while low expression in MCF-7 cells. Recapitulated PIAS4 NMR levels in MCF-7 human breast cancer cells by overexpressing after which it downregulates anti-apoptotic protein Bcl-2. Exposure to doxorubicin (DOX)-induced cell death which is further enhanced by PIAS4 expression. PIAS4 overexpression caused a in decrease colony formation and cell invasion. PIAS4 may enhance DNA repair mechanisms, supporting cellular adaptation under genotoxic stress

Article Snippet: Non-malignant breast epithelial cells (MCF-10 A) at passage 3 (P3) and human breast cancer cells (MCF-7) at passage 6 (P6) were provided by the Institute of Cancer Therapeutics (ICT) at the University of Bradford (UoB) originally sourced from ATCC.

Techniques: Expressing, Over Expression